FIA Solutions
Fluorescence immunoassay (FIA) is a simple, rapid, and sensitive technique that is used to quantitevly mesure many compounds including hormones, proteins and drugs. This method has been widely applied in the in vitro diagnostics (IVD) industry.
A modern fluorescent based immunoassay uses as the detection reagent – fluorescent microspores which absorb light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.
The FIA test consists of:
1) a conjugate pad containing specific protein (antibody or antigen) conjugated with fluorescent microspheres (probe conjugate) and a control antibody robe conjugate.
2) a nitrocellulose membrane with immobilized proteins in the test area (T line) and a control line (C line).
After adding the specimen to the sample well on the test cassette, protein in the sample will interact with the probe conjugate on the probe pad. The NC membrane diffuses re-solubilized conjugate forward. As the sample moves along the devise binding reagents situated on the NC membrane bind to the target at the T and C line.
Concentration in specimen is positively correlated with the fluorescent density of the fluorescent microspheres, after the measure and calculation of the quantitative fluorescence immunoassay analyzer, then the analyzer would calculate the concentration according to the preset calibration curve in the machine and display the result. C line should appear in any cases, which indicates the reaction system is effective.
As an expert in IVD assay development, AIVD Biotech is committed to offering a reliable and sensitive FIA-based testing service to accelerate the application of FIA technique in the IVD field.
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Item |
Catalog # |
Linear Range |
Linearity |
CorrelationR2 |
Detection limit |
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cTnI |
IFCNI001/ IFCNI100 |
0.1~40ng/ml |
0.9995 |
0.969 |
0.0045ng/ml |
|
Myo |
IFMYO001/IFMYO100 |
25-500ng/ml |
0.9999 |
0.9338 |
15ng/ml |
|
CK-MB |
IFCKB001/IFCKB100 |
1-100ng/ml |
0.9999 |
0.93 |
0.4ng/ml |
|
NT-pro BNP |
IFNTP001/ IFNTP100 |
25-35000pg/ml |
0.9993 |
0.979 |
25pg/ml |
|
D-dimer |
IFDDT001/IFDDT100 |
0.1-10mg/L |
0.9998 |
0.95 |
0.05mg/ml |
|
h-FABP |
IFABP001/ IFABP100 |
2.5-160ng/ml |
0.9996 |
0.93 |
1ng/ml |
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Product Name: Benchtop Fluorescence Immunoassay Analyzer Model # : AI-IF 600 Wave length: Excitation wavelength of 365nm, absorption of 610nm. Features:1.Can be operated in batches rapidly 2.One-click operation, card ejects automatically 3.Identify the test item automatically 4.Automatically print and upload the data 5.High sensitivity, reach to nanogram level |
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Service Features: |
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Service Contents: |
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1. OEM service for diverse parameters and performance requirements. 2. Raw materials and semi-finished products: proteins, uncut sheets, OEM test kits 3. Custom services for different platform applications and performance requirements. 4. Personalized product and collaborative development, strategic cooperation among enterprises, technology sharing model. |
1. Raw materials development and production 2. Optimization of raw material coupling process system 3. Optimization of production process 4. Guidance for production platform and personnel training 5. Production of bulk reagents and uncut sheets 6. Development for new products 7. Technical transfer |
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Contact us for more informations
Email (General Inquiry, Technical Support & Feedback): info@aivdbiotech.com
Email (Placing Order & Sales Support): market@aivdbiotech.com
Tel: +86 (755)26165742
WhatsApp, WeChat, Tel: +86 18543132823
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Immunoassay is a highly selective bioanalytical method that measures the presence or concentration of analytes including macromolecules and small molecules in a solution through the use of an antibody or an antigen as a biorecognition agent. This method is based on the specificity of antibodies to their antigen. Fluorescent probes, as detectable signals, have been widely used in immunoassay. Fluorescent probes have many advantages as detectable signals including high sensitivity and a variety of measurable properties such as emission intensity, orientation, waveform, and lifetime. In a classical FIA, fluorescent dyes that illuminate in UV light are used to detect the specific binding of an antigen and antibody. The advantages of an FIA method include higher sensitivity detection of the analyte, simplified reagents, and simpler assay designs.
Fluorescence immunoassay (FIA) is a simple, rapid, and sensitive technique that is used to quantitevly mesure many compounds including drugs, hormones, and proteins. This method has been widely applied in the in vitro diagnostics (IVD) industry. As an expert in IVD assay development, AIVD Biotech is committed to offering a reliable and sensitive FIA-based testing service to accelerate the application of FIA technique in the IVD field.
